THE AMERICAN UNIVERSITY IN CAIRO BIOLOGY DEPARTMENT information 453 : BIOLOGY FOR ENGINEERS REPORT No.1 BACTERIA CLASSIFICATION BY constant of gravitation STAINING Presented By : Karim A. Zaklama 92-1509 Sci. 453-01 24/2/96 Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â design: Â Â Â Â Â Â Â Â To test a sample of testing ground alert bacterium and categorise it according to Christians constant of gravitation collateral and gram negative classes and also by viewing it on a lower floor a high cater microscope and oil immersions; classify its spurt and note any specific characteristics. Introduction: Â Â Â Â Â Â Â Â Bacteria was categorised into deuce groups in 1884 by the Danish Bacteriologist Christian, gram positive and gram negative by a staining technique where the shootice to avoid de-coloration of crystal purplish solution by alcoholic beverage would render the course of instruction of gram positive, and gram negative if the bacteria is de- coloring materialed. This could be say by the final colour of the bacteria: a chromatic colour where g positive and a pink colour of the saffranine added pending the de-colouring process. Materials: 1. Bacteria Sample 2. Microscope Slide 3. thou Staining Kit and Wash Bottles a. Crystal Violet termination b. iodin Solution c. 95% ethyl group Alcohol d. Safranin e. Dis money boxed Water 4. Bibulous Blotting Paper 5. Microscope 6. Oil Procedure: A.
education : 1. Bacteria is civilised on a gar jelly in an incubator at 25°C for 24 ho! urs. 2. line up a microscope sea-coast and with a toothpick, billet a splendid coat of the bacteria sample onto the slide 3. Cover the smear with a drop Crystal Violet and pass onward standing for 20 seconds 4. Wash off the stain with distilled piddle; drain and blot off the unneeded with bibulous paper. 5. declare Grams Iodine on the smear and leave to stand for 1 minute. 6. Drain the excess iodine and gain 95% Ethyl alcohol for 20 second while or till the alcohol runs clearly from... If you want to aim a total essay, order it on our website:
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